Topic: cryo-electron tomography

The workshop integrates cryo-electron tomography, fluorescence microscopy, mass spectrometry and computational modeling to investigate macromolecular complexes within intact biological systems. Sessions connect molecular machines with organelles, whole cells and multicellular interactions, emphasizing complementary data and methods that preserve native context. It welcomes experts and early-career life scientists interested in advanced imaging and system-wide analysis. On-site sessions start at 13:30 on 9 February and finish at 12:30 on 12 February, Europe/Berlin time. On-site registration clo

In situ cryo-electron tomography can reveal interactions between pathogens and their hosts inside intact cellular environments. This seminar uses apicomplexan parasites and gram-negative bacteria to explain the workflow from sample preparation through image acquisition and analysis. It covers freezing, cryo-focused ion beam milling, cryo-electron tomography, correlative light and electron microscopy, tomogram reconstruction, segmentation and subtomogram averaging. The presentation discusses which aspects of the host-pathogen interface these approaches can resolve, their advantages and limitati

The brain is complex over multiple length-scales, from many protein molecules forming intricate nano-machines in a synapse to many neurons forming interconnected networks across the brain. Unraveling this multi-scale complexity is fundamental to our understanding of brain function and disease. In this lecture, I will introduce advances in visualizing the complex, multi-scale structures in the brain. Emphasis will be on new imaging techniques, including cryo electron tomography and correlative light-electron microscopy that enabled revealing in situ organization of synaptic molecules, and ultra

Neurons communicate at specialized terminals called synapses, where hundreds of molecules work together in harmony to ensure proper and efficient signal transmission, which is crucial for normal brain functions. Aberrant synaptic signalling is involved in many neurodevelopmental and neurodegenerative diseases such as autism and Parkinson’s disease. This highlights the need to understand the organization and interactions of molecular complexes at the synapse. Cryo-electron tomography (cryo-ET) offers a close-to-native preservation of biological specimen and the unique possibility to capture bot