Topic: dopaminergic neuronal loss

We aimed to develop and standardize a diet and rotenone-based animal model of Parkinson's disease. To achieve this, 21 days old male C57BL/6 mice were fed on a high-fat diet [HFD] (60%kcal) for 17 weeks; starting from week 11, animals were orally gavaged daily with 10mg/kg rotenone dose till the end of the 17th week. Further, they were subjected to behavioral tests (Rota-rod, Y maze, and fecal pellet output test). Fecal pellets were collected for 16S rRNA sequencing and animals were subsequently euthanized. Our data revealed that rotenone and its combination with HFD exhibited loss of motor fu

Astrocytes are essential for brain health. Indeed, they provide structural and metabolic support; regulate synaptic transmission; maintain glutamate homeostasis as well as produce neurotrophic molecules. In Parkinson’s disease (PD) these pathways became progressively dysfunctional thus switching to a neurotoxic phenotype. Scientific evidence suggests that neurotoxic glial cells might be responsible for neuroinflammation that plays a key role in the symptoms and development of PD. However, the mechanistic correlation among glial activation, neuroinflammatory response, dopaminergic neuronal loss