Topic: dry eye disease

Dry eye disease (DED) affects 30% of the adult population worldwide and causes corneal pain which remains a therapeutic challenge. Our study aimed to identify the first brain connectome associated with corneal pain by mapping c-Fos positive cells (an indirect marker of neuronal activation) in the whole mouse brain.Adult male C57BL/6 mice received unilateral topical instillation of 0.2% benzalkonium chloride twice/day for 7 days (DED model, n=5) and were compared to naive animals (n=5). Spontaneous ocular pain (eye closure ratio) and corneal mechanical sensitivity (von Frey test) were monitored

Dry eye disease (DED) involves neurosensory abnormalities and inflammation. The aim of this study was to elucidate the role of transient receptor potential ankyrin-1 (TRPA1), a highly expressed corneal nociceptor, in neurogenic inflammation associated with persistent DED.Multi-unit extracellular recording of naive mice ciliary nerve was monitored with/without TRPA1 antagonist (HC030031), to determine which corneal nerve fiber is inhibited by this antagonist. DED was induced in mice by removing the extraorbital lacrimal gland and Harderian gland. Mice underwent topical treatment with HC030031 o