Topic: fluorescent probes

Neuromodulators such as monoamines or neuropeptides play crucial roles in modulating diverse brain functions. They can be released phasically, i.e. in response to stimuli, or tonically, in a spontaneous and more discrete manner. Understanding how these spontaneous releases occur is challenging yet essential, as their disruption is associated with behavioral and psychiatric disorders. A paradigm shift in this domain has been the generation of fluorescent probes sensitive enough to detect, using 2-photons microscopy, discrete tonic release of specific neuromodulators including dopamine, noradren

Progress in brain research is widely based on experimental approaches having the potential to advance the knowledge on fundamental biology. In particular, the increasing availability of data obtained by experimental techniques has boosted the development of data-driven mathematical models reproducing neuronal circuits. While cellular and subcellular resolution data have been extensively collected in animal models, experimental constraints have limited the acquisition of information about human neuronal cytoarchitectures and morphologies. In this context, high resolution data can be efficiently