Topic: forced swim test

The main objective of the study was to investigate the antidepressant like effect of curcumin in olfactory bulbectomized model of depression of male wistar albino rats through behavior screening tests. Bilateral olfactory bulbectomy was performed with rats anesthetized under ketamine (50 mg/kg/i.p.).Twenty male albino wistar rats, weighing 150–220 gm were randomly allocated into four groups (n=5)- Group A (control), Group B(olfactory bulbectomy;OB), Group C {vehicle (0.9% NS, 10 ml/kg) +olfactory bulbectomy,OB} & Group D {curcumin (40 mg/kg)+ olfactory bulbectomy;OB}.Behaviour screening te

Introduction: Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel expressed in the urocortin 1 (UCN1) positive neurons of the centrally projecting Edinger-Westphal nucleus (EWcp) having modulatory impact on stress adaptation. As post-traumatic stress disorder (PTSD) is triggered by disturbed stress adaptation, we hypothesized the regulatory role of EWcp/UCN1/Trpa1 neurons in PTSD.Methods: Male TRPA1 wild-type (WT) and knockout (KO) mice were exposed to two different models of PTSD. In single prolonged stress (SPS) paradigm, time spent immobility was measured during

Many neuroscience experiments involve manual scoring of animal behavior by a human operator. This operation is time consuming, is liable to bias and hinders reproducibility. For these reasons, automatic systems to score animal behavior from videos have gained increasing attention in recent years. Here, we present a machine learning approach to automatically score behavior in the Forced Swim Test (FST). During FST, animals perform three different behaviors: climbing, swimming and immobility. So far, these behaviors have been evaluated by an operator (a bias-prone method) or using automatic syst

The first and probably most important bond is between a mother and her infant promoting their psychological and physiological well-being. Here, the “pro-maternal” oxytocin system as well as the “anti-maternal” CRF system are strongly involved in the formation and maintenance of this maternal bond. Losing a child is a severely stressful experience that dramatically affects the maternal brain, and those neuropeptide systems are promising candidates concerning the underlying emotional and behavioral consequences. Our study endeavored to investigate the changes in rat mothers' CRF system activity

Major Depressive Disorder (MDD) poses a global burden, affecting over 300 million individuals and presenting a significant therapeutic challenge due to the limited efficacy of conventional antidepressants. The investigation of rapid-acting antidepressants, exemplified by ketamine, has garnered attention, yet the intricate underlying processes remain incompletely understood.This study systematically evaluated the acute effects of ketamine (10 mg/kg, i.p.) and fluoxetine (3 x 10 mg/kg, i.p.) on depression-like behavior in animal models subjected to chronic restraint stress (CRS, 6 weeks) and chr

Drug addiction poses a significant public health challenge, impacting individuals and societies at large. While substantial progress has been made in understanding the neurobiological underpinnings of addiction, certain aspects remain unresolved. Satb2 is a DNA-binding protein involved in gene expression regulation through chromatin remodelling. We propose that it can emerge as a potential player in addiction mechanisms. A Satb2 conditional mutant (cKo) (Satb2 flox/flox::Camk2a-cre) that was generated showed impairment in long-term memory and an absence of this gene in the Cortex and CA1 regio

The effect of living conditions on the physiology, behaviour and stress of laboratory animals is still being discussed. There are conflicting findings regarding individually ventilated cages(IVC). This study aims to explore the effects of three distinct cage setups and to examine the differences in the impacts of IVC cages, classical cages and classical cages with varying mouse populations. Thirty male C57BL/6J strain mice were split into three groups:IVC, classical small(CC500) and big(CC800) with a floor size of 500 and 800 cm2,respectively. From birth until six months of age, the mice were

The prenatal microbiome influences offspring health, by potentially altering postpartum maternal behavior. Identified as microbiome disruptors, prenatal stress and gestational antibiotic administration pose risks to maternal and offspring health.We conducted two studies exploring the impact of 1.Antibiotic administration and 2.Stress during gestation on postpartum maternal behavior using Wistar rats. Additionally, we examined the potential benefits of a multi-strain probiotic intervention.In the first study, a subgroup of pregnant rats received an antibiotic cocktail (ampicillin+vancomycin+neo