Topic: functional ultrasound

Functional ultrasound (fUS) imaging has recently allowed 3D functional imaging in awake mice using motorized linear or matrix arrays (Brunner et al., 2020; Bertolo et al., 2021). However, the tradeoff between the field of view and temporal resolution introduced by motorized scanning hinders acquiring whole-brain resting state Functional Connectivity (rsFC), whereas the limited sensitivity of matrix arrays prevents transcranial imaging in mice. Here we propose a new hybrid approach dedicated to 3D rsFC in the whole mouse brain with intact skull.A newly developed 15 MHz multiarray probe (IcoPrim

Episodic memories become gradually independent from the hippocampus and progressively consolidate in the neocortex. During sleep and rest periods, neuronal traces are reactivated at the time of hippocampal sharp-wave ripples (SWRs) thought to mediate memory consolidation. Though SWRs have been widely studied within the hippocampus, the ongoing activity within the rest of the brain remains elusive, because technically challenging: electrophysiology cannot easily resolve the whole brain and optical recording techniques have limited access to deep tissue. In a 2012 study, Logothetis and colleague

Delayed Cerebral Ischemia (DCI), is a complication of sub-Arachnoïdal Hemorrhage (sAH) associated with a high mortality and morbidity, which is responsible for heavy impairments on survivors. DCI is a slow developing pathology and its diagnosis requires concomitant monitoring of functional or motor deficits and brain perfusion or lesions. In our study we aim, after the development and validation of a preclinical DCI model, to investigate the utility of functional ultrasound (fUS) imaging for longitudinal monitoring of DCI. First we have adapted and refined a rodent model for the minimally-inva

This study investigates brain functional network dynamics of freely behaving but head-fixed mice using functional ultrasound (fUS). Unlike traditional fMRI, fUS allows recording brain activity in awake, behaving mice without anesthesia. We established a pipeline for processing fUS data and applied it to behavioral mouse data from a preclinical model of autism carrying a Shank2 gene knockout mutation. The pipeline includes several pre-processing stages, a deconvolution-based method for investigating neuronal activity underlying the fUS signal, and an analysis using hidden Markov models (HMM) to

Aims. Psychedelics produce fast and persistent antidepressant effects and induce neuroplasticity resembling the effects of clinically approved antidepressants. We have recently found that lysergic acid diethylamide (LSD) directly bind to TrkB, the receptor for BDNF. Our data suggest that antidepressant and plasticity promoting-effect of psychedelics are mediated by TrkB. Our objective is to test in vivo the effect of LSD on brain functional connectivity, more specifically on the default mode network, and the impact of the modulation of TrkB receptor on this effect. Methods. Functional Ultrasou

Traumatic brain injury (TBI) is the leading cause of death in the pediatric stage, with detrimental consequences including psychosocial issues, cognitive deficits and motor abnormalities. In addition to the primary TBI impact, the subsequent neuroinflammatory response can become a contributing and even leading factor to the harmful effect of pediatric TBI on the brain. As such, finding ways to stabilize the reactive microglial phenotype is a key objective for TBI treatment. One potential factor is the neuropeptide oxytocin. Oxytocin has shown to be neuroprotective in adult neuroinflammatory st