Topic: GAD67

The marginal zone (MZ) is the most superficial layer of the developing telencephalon which will become cell sparse adult cortical layer I. In this study the MZ in human and cynomologus monkey was examined on Nissl and immunohistochemically processed postmortem fetal brain tissue. From 7 to 13 postconceptional week (pcw) in human, and from embryonic day (E) 46 to 56 in monkey, large cells that express calbindin and calretinin were observed in the MZ, but the overall cell density was exceptionally low. At 15pcw and E64 cell density increased, and small cells that express calretinin, calbindin, s

Although the aetiology of schizophrenia is still not thoroughly understood, post-mortem studies of patients have consistently revealed alterations of GABAergic interneurons, particularly lower levels of GAD67 and parvalbumin in cortical parvalbumin interneurons (PVs). A deficient maturation of PVs could relate to the neurodevelopmental aspect of schizophrenia since normal wiring of cortical circuits relies on this process. We have previously shown that mice with an early postnatal NMDA receptor ablation in corticolimbic GABAergic interneurons –predominantly PVs– present schizophrenia-like phen

Posttraumatic stress disorder (PTSD) manifests in some trauma-exposed individuals through excessive fear generalization to safe contexts and difficulties extinguishing fear. While previous studies have implicated functional changes in fear-regulatory nodes a comprehensive understanding of network-wide alterations remains elusive. We employed a rodent model of PTSD to investigate whole-brain activity patterns associated with fear generalization. Male rats underwent uncontrollable footshocks and 28 days later we measured their generalized fear response in an altered/safe context. Based on freezi

Thoracic complete Spinal Cord Injury (Th-SCI) produces physiological changes in the hindlimb somatosensory cortex (S1HL), which we have characterized as neuronal network hyperexcitability. Moreover, we have found a decreased GAD67 labeling intensity in the infragranular layers of S1HL in chronic Th-SCI. However, it is unknown how decreased labeling of GAD67 could be related to altered inhibitory function of GABAergic interneurons across cortical layers after SCI. The present work has two aims: characterize the physiological effect of a chronic Th-SCI over the different GABAergic neuronal popul

Dynamic reorganization of neuronal circuits in the spinal dorsal horn is assumed to cause sensitization in the neuropathic pain. In our previous study, brain-enriched guanylate kinase associated-protein (BEGAIN) was identified as an increased protein in the spinal dorsal horn in the neuropathic pain and the mechanical allodynia was attenuated in the BEGAIN-knockout mice. BEAGIN-expressing neurons have not yet been identified because BEGAIN protein is detected in highly localized dendritic regions, not in the cell body. To identify BEGAIN-expressing neurons, double in situ hybridization with RN