Topic: GCaMP8m

Working memory is a fundamental cognitive brain function enabling us to hold transiently and process sensory information for a future goal. The prefrontal cortex was long thought to be the specific site for working memory in mammals. However, there is growing evidence that parallel distributed circuits may be responsible for working memory stressing the importance of sensory cortices for the active maintenance of information in working memory. The specific functions of working memory representations in sensory versus associate areas remains unknown. One possibility is that associative areas co

The increasing prevalence of psychiatric disorders, such as depression, highlights the need to elucidate their underlying neurobiological mechanisms. The lateral habenula (LHb) and its neuronal activity contribute to the pathophysiology of depression when dysfunctional. Among the existing rodent models of depression, chronic corticosterone administration for 3-4 weeks recapitulates relevant behavioural aspects of the disease such as anhedonia and increased passive coping. However, the temporal emergence of these features and the cellular underpinnings remain unclear. Thus, we assessed the emer

Aims. Though electrophysiological methods allow recording neuronal activity from freely moving animals, the localization and type of cells recorded can only be deduced from indirect markers. Two-photon microscopy can provide exact localization and immediate identification of cell types. Our aim was to develop and test an imaging modality that is capable of deep-tissue long-term two-photon imaging while being light and compact for a mouse to carry while freely moving in its environment.Methods. All experimental procedures were approved by the French ethics committee (APAFiS #30959) and were con