Topic: glioblastoma stem cells

Glioblastoma (GBM) patients have a poor prognosis due to glioblastoma stem cells (GSCs) that resist current treatments. Leucine-rich repeats and immunoglobulin-like domains (LRIG) proteins, particularly Lrig1, play a role in growth factor signaling in various stem cells. In this study, the function of Lrig1 in glioblastoma stem cells was investigated using gain- and loss-of-function experiments. Removing Lrig1 in cultured GBM stem cells increased proliferation and reduced quiescence, while overexpression of Lrig1 enhanced quiescence and reduced proliferation. Mice transplanted with Lrig1-defic

Glioblastoma multiforme (GBM) is a highly therapy-resistant primary brain tumor, especially due to the presence of Glioblastoma Stem Cells (GSCs). In fact, GBM treatment imposes a combination of brain surgery, radiotherapy and chemotherapy with Temozolomide (TMZ) but still fails to completely abolish the tumor. Given this, exploring differentiation induction in GSCs emerges as a promising strategy for GBM treatment. Accordingly, we aimed to investigate the mechanisms that regulate stemness maintenance and differentiation in GBM using Whole-Cell Patch Clamp, Western Blot, Immunofluorescence and

Glioblastoma is the most common and aggressive primary brain tumour. Cancer hijacks developmental pathways and several studies have identified a stem cell-like population in glioblastoma following neurodevelopmental trajectories. In the foetal neocortex, a type of neural stem cell called basal progenitor is thought to be responsible for neocortical expansion. Basal progenitors exist in different morphotypes, and greater morphological complexity underlies their increased proliferation. We thus propose that cell morphology also plays a role in the proliferation of glioblastoma stem cells. Throug

Glioblastoma, a highly aggressive brain tumor, poses a formidable challenge due to factors such as angiogenesis, diverse mutations, and evasion of apoptosis. Nitric oxide (NO) plays multifaceted roles in physiological processes, including vasodilation, blood pressure regulation, neurotransmission, and immune response. In the context of glioblastoma, understanding the interaction between NO and tumor cells is crucial. Our study utilizes human and mouse glioblastoma stem cells to investigate how NO influences cell proliferation.We modulate NO activity by either enhancing (using SNAP) or inhibiti