ePoster · Neuroscience
Kseniia Morozova, Daria Kotova, Aleksandra Ivanova, Matvei Pochechuev, Ilya Kelmanson, Yulia Khramova, Alisa Tiaglik, Mark Sudoplatov, Arina Trifonova, Anna Fedotova, Veronika Katrukha, Anastasia Sergeeva, Roman Raevskii, Mariia Pestriakova, Maxim Solotenkov, Evgeny Stepanov, Aleksandra Tsopina, Aleksandr Moshchenko, Milena Shestopalova, Anton Zalygin, Ilya Fedotov, Andrei Fedotov, Vladimir Oleinikov, Vsevolod Belousov, Alexey Semyanov, Nadezda Brazhe, Aleksei Zheltikov, Dmitry Bilan · FENS Forum 2024
Diabetes has a systemic effect on the metabolism and can be linked to higher risks of severe medical conditions, such as ischemic stroke. Hyperglycemia leads to more extensive cerebral damage during stroke and, as a result, to more severe consequences. One of the main damaging factors of the stroke is the reactive oxygen species generation occurring in mitochondrial respiratory chain (electron transport chain, ETC). We used in vivo Raman microspectroscopy with the chronic cranial windows implantation to assess the redox state of mitochondria ETC in neurons of awake mice under normal and hyperg
ePoster · Neuroscience
Alisa Tiaglik, Ksenia Morozova, Anna Fedotova, Milena Shestopalova, Anton Zalygin, Vladimir Oleinikov, Dmitriy Bilan, Nadezda Brazhe, Alexey Semyanov · FENS Forum 2024
Neurons and astrocytes, key elements of the brain active milieu, differ in the metabolic profile and the electron transport chain (ETC) organization. We assessed the redox state of the ETC in two cell types with simultaneous analysis of blood oxygen saturation level (sO2) in awake mice with Raman microspectroscopy. For cell identification, green fluorescent protein (GFP) was expressed in astrocytes, and near-infrared protein (NirFP) was expressed in neurons following AAV injections in the somatosensory cortex (S1). To monitor H2O2, HyPer7 was expressed in the mitochondria of either astrocytes
ePoster · Neuroscience
Theresa Gehling, Filip Liebsch, Lianne Jacobs, Jan Riemer, Günter Schwarz · FENS Forum 2024
Reactive oxygen species (ROS) play a central role in enhancing inhibitory signal transmission, thus extending their role beyond oxidative stress in disease and aging. However, the underlying molecular mechanisms mediating these functions have remained elusive. At inhibitory synapses, the scaffolding protein gephyrin clusters glycine and GABA type A receptors. Since gephyrin harbors multiple surface-exposed cysteines, we investigated the regulatory influence of ROS on gephyrin. We show that H2O2-induced oxidation of gephyrin cysteines triggered reversible, synaptic multimerization through disul