Topic: hydrogen sulfide

The gene for cystathionine beta-synthase (CBS), the enzyme producing hydrogen sulfide (H2S), located on chromosome 21, is present in extra copy in Down syndrome (DS). Dysregulation of H2S signalling has been linked to DS, but the precise underlying mechanisms are yet to be fully identified. Therefore, this study aims to establish and characterize induced pluripotent stem cell (iPSC) lines reprogrammed from peripheral blood mononuclear cells (PBMC) collected from a blood sample of a paediatric DS patient (T21) and an age-matched apparently healthy donor (Eup), and also to derive iPSC into neura

Peripheral nerve degeneration (PND) regulated by Schwann cells in a hydrogen sulfide (H2S)-pathway-dependent manner is well known, but the underlying molecular properties of Schwann cells during PND are unclear. We investigated H2S signaling in in vitro- and ex vivo-dedifferentiated Schwann cells as unipotent stem cells by using global proteomics (LC-MS) and transcriptomics (whole-genome and small RNA-sequencing [RNA-seq]) methods. The multi-omics analyses identified several genes and proteins related to oxidative stress, such as Sod1, Gnao1, Stx4, Hmox2, Srxn1, and Edn1. The responses to oxid