Topic: in vivo 2-photon calcium imaging

Transcriptomics has revealed the exquisite diversity of cortical inhibitory neurons, but it is not known whether these fine molecular subtypes have correspondingly diverse activity patterns in the living brain. Here, we show that inhibitory subtypes in primary visual cortex (V1) have diverse correlates with brain state, but that this diversity is organized by a single factor: position along their main axis of transcriptomic variation. We combined in vivo 2-photon calcium imaging of mouse V1 with a novel transcriptomic method to identify mRNAs for 72 selected genes in ex vivo slices. We used tr

Working memory is a fundamental cognitive brain function enabling us to hold transiently and process sensory information for a future goal. The prefrontal cortex was long thought to be the specific site for working memory in mammals. However, there is growing evidence that parallel distributed circuits may be responsible for working memory stressing the importance of sensory cortices for the active maintenance of information in working memory. The specific functions of working memory representations in sensory versus associate areas remains unknown. One possibility is that associative areas co