Topic: interstitial fluid

Orthotopic xenograft studies strongly promote the development of personalized and targeted cancer therapies to increase the still very poor life expectancy of glioblastoma (GBM) patients. As the tumor microenvironment (TME) plays an active role in cancer progression, the TME has increasingly become subject of investigations recently.We aimed to assess the metabolome from interstitial fluid (ISF) collected with Open Flow Microperfusion (cOFM) from the TME and compare it to the metabolome from cerebrospinal fluid (CSF). This was done via atraumatic access that has been generated by inoculating m

Neurons and glial cells are surrounded by interstitial fluid (ISF), which primarily originates from cerebrospinal fluid (CSF). The fluid dynamics of ISF and CSF have been examined by cisterna magna injection of fluid tracers such as fluorescent dextran or contrast agents for magnetic resonance imaging. While this approach has unveiled major CSF flow dynamics in the recent decade, cisterna magna injection introduces transient intracranial pressure changes that can lead to experimental variability. In this study, we attempted to label the CSF/ISF via endogenously expressed secretory fluorescent