Cerebrospinal fluid

…the metabolome from interstitial fluid (ISF) collected with Open Flow Microperfusion (cOFM) from the TME and compare it to the metabolome from cerebrospinal fluid (CSF). This was done via atraumatic access that has been generated by inoculating m

…that make up the brain extracellular fluid are not secreted locally at the brain, but rather synthesized in peripheral organs and then imported to the nervous system via the cerebrospinal fluid (CSF) or the bloodstream. Despite the relevance of the ISS during brain development, profiling the different molecules that compose the extracellular space of the brain has proven to be a daunting task due to the complexit

…Neurones in contact with the cerebrospinal fluid (CSF-cNs), localized around the medullo-spinal central canal in all vertebrates, are GABAergic and selectively express PKD2L1 channel (Polycystin Kidney Disease 2-like1), a member of TRP superfamily, with chemo/mechanosensitive properties. In zebrafish, CSF-cNs respond to spinal cord bending and modulat

…in the spinal cord that contact cerebrospinal fluid (CSF) across vertebrate species. These neurons, called spinal CSF-contacting neurons (CSF-cNs), modulate locomotion and posture by detecting mechanical and chemical cues from the CSF. However, the mechanisms by which CSF-cNs detect and respond to CSF changes are unknown. In this study, we investigate whether CSF-cNs in larval zebrafish can detect and respond to pathological CSF changes induced by a ventricular injection of Streptococcus pneumoniae (S. pneumoniae), the major pathogen

Choroid plexus (ChP) is located in cerebral ventricles, where it produces cerebrospinal fluid (CSF), forms the selective blood-CSF barrier and controls the homeostasis of the central nervous system. ChP is primarily composed of a monolayer of specialized epithelial cells producing cerebrospinal fluid. Differentiated ChP epithelial cells harbor multiple cilia on the CSF-facing surface, but their development and function are still controversial. We have delineated the sequential progression of cilia formation in the ChP epithelial cells by exploiting super-resolution microscopy techniques and mo

Cerebrospinal fluid (CSF) is a clear, colorless fluid that bathes the central nervous system and is essential for brain homeostasis. CSF flows through interconnected ventricles which are lined by ependymal cells that generate a directional flow upon beating of their motile cilia. Defects in motile cilia can lead to a variety of neurological conditions like hydrocephalus, which is characterized by an accumulation of CSF in the brain. Nevertheless, the function of motile cilia-mediated fluid flow and its impact on brain development and physiology is still poorly understood. To address these ques

…the production of a large part of the cerebrospinal fluid (CSF). In addition, the epithelial cells of the ChP synthesize neurotrophic factors and other signalling molecules into CSF and act as a blood-cerebrospinal fluid barrier. Importantly, ChP cells harbour a robust circadian oscillator that may control ChP function and may play an important role in the circuitry between brain clocks. In this study, we aimed to find out whether the ChP clock is sensitive to a robust imm

…Biofluids, such as serum and cerebrospinal fluid, contain miRNAs that can be identified and quantified. The project aims to identify circulating miRNAs within serum and CSF, which can be used as non-invasive

…the delivery of antibodies in the cerebrospinal

…global grey matter (gGM-fMRI) and the cerebrospinal fluid signal in the fourth ventricle (CSF-fMRI) is thought to reflect macroscopic glymphatic fluid movement, suggesting relevance for brain clearance. However, direct evidence linking gGM-CSF coupling with brain clearance measures is still missing. We hypothesized that in humans, gGM-CSF coupling is associated with two other measures of the glymphatic system,

The choroid plexus produces cerebrospinal fluid (CSF) by transporting ions and water from adjacent blood vessels to the brain ventricles. The choroid plexus plays additional roles in brain development and homeostasis by secreting neurotrophic molecules and serving as a CSF-blood barrier and immune interface. Previous studies highlighted that several transporters on the epithelial cells transport water and ions into the ventricles and tight junctions of the cells are involved in the CSF-blood barrier. Yet, how the choroid plexus epithelial cells maintain the brain ventricle system and influence

…levels were measured in 46 sera and 20 cerebrospinal fluid (CSF) samples from 13 HLA-subtyped anti-IgLON5 disease patients (6 female, 7 male) using flow cytometry. Intervals between two consecutive serum or CSF samplings were analyzed for treatment, changes of anti-IgLON5 IgG1/IgG4 levels and disease severity. Intrathecal anti-IgLON5 IgG4 synthesis (IS) was assessed. Th

Phenotypic and compositional changes of immune cells in cerebrospinal fluid (CSF) can be used as biomarkers to help diagnose and track disease activity for neuroinflammatory and neurodegenerative diseases, such as multiple sclerosis (MS) and Alzheimer´s disease (AD). The CSF is the compartment in closest proximity to the central nervous system (CNS) with unique immune cell composition and routine CSF collection is integral for diagnosing various CNS disorders, offering a more practical alternative to assess the CNS. In this study, we described an end-to-end workflow to perform in-depth single-

Cerebrospinal fluid (CSF) is integral to brain architecture, providing mechanical and nutritional support to the tissue while clearing metabolic waste. Recent research reveals its potential role in neuromodulation through its transport of various signaling molecules. Yet, to what extend CSF impacts the direct delivery of such molecules is poorly understood. CSF circulation is tightly regulated by a combination of factors such as cardiac activity, glial regulation, and notably, ciliary beating of ependymal cells lining the brain ventricles. Ciliary dysfunction, as observed in ciliopathies, ofte

…is dependent on the directed flow of fluid across different compartments in the brain and associated lymphatic vessels. The coupling between the coherent global grey matter activity (gGM-activity) and cerebrospinal fluid movement (gGM-CSF-coupling) is thought to drive glymphatic fluid movement in humans. Since gGM-coherence is severely impaired in patients with schizophrenia, the glymphatic system might be impaired in the disorder. Therefore, we hypothesized aberrant glymphatic function in schizophr

…kit was developed and tested in 89 cerebrospinal fluid (CSF) samples of mild cognitive impairment (MCI) and Alzheimer’s disease (AD) patients for detection of p-Tau.The proof of concept study reported specific signals above the

…patients, the levels of irisin in the cerebrospinal fluid (CSF) and plasma, and their correlations with disease biomarkers. Biological samples were obtained from patients with Alzheimer's dementia (n=82), mild cognitive impairment (n=44) and subjective memory complaint (n=20) biologically character

…for improved diagnostic precision.Cerebrospinal fluid (CSF) and serum samples from 24 healthy controls, 48 MS patients, and 20 Non-MS patients with a range of diverse conditions, including infections, autoimmune disorders, inflammatory issues, and other neurological disorders were collected during lumbar puncture. NMR analysis on a

…can be released from the brain to the cerebrospinal fluid (CSF). Their presence in the CSF was described using electron microscopy, PAS staining and immunofluorescence with natural IgMs and anti-glycogen synthase (GS) antibodies. However, the methods employed involved the use of a large sample volume for each staining, which hindered wasteosomes characterization. In the present work, we examined a new method to

Hydrocephalus is a disorder that involves accumulation of cerebrospinal fluid (CSF) in the ventricles of the brain. The incidence is significantly higher in preterm neonates, which is highly associated with intraventricular hemorrhage (IVH). The sequela of severe IVH manifests post-hemorrhagic hydrocephalus (PHH) that contributes to neurologic morbidity and mortality in preterm infants. There have been numerous animal models to study the mechanism and progression of hydrocephalus since 1913. However, we still lack an IVH-induced hydrocephalus model that accurately reflects the gestational age

Neurons and glial cells are surrounded by interstitial fluid (ISF), which primarily originates from cerebrospinal fluid (CSF). The fluid dynamics of ISF and CSF have been examined by cisterna magna injection of fluid tracers such as fluorescent dextran or contrast agents for magnetic resonance imaging. While this approach has unveiled major CSF flow dynamics in the recent decade, cisterna magna injection introduces transient intracranial pressure changes that can lead to experimental variability. In this study, we attempted to label the CSF/ISF via endogenously expressed secretory fluorescent

…Serum and cerebrospinal fluid (CSF)

Liver-secreted albumin is the most abundant protein in blood plasma and cerebrospinal fluid. We have previously developed liver-targeting adeno-associated viral vectors (AAV8-P3-*) that express fluorescent protein-tagged albumin to visualize blood plasma in adult mice (DOI: 10.1016/j.crmeth.2022.100302). We have also established a virally induced CRISPR/Cas9-based knock-in of green fluorescent albumin in neonatal mice (DOI: 10.1101/2023.07.10.548084). Here, we have generated a new transgenic mouse model by CRISPR/Cas9 in which the bright red fluorescent protein mScarlet is knocked into the alb