Topic: cerebrospinal fluid

Multiple Sclerosis (MS) is a chronic inflammatory disorder of the central nervous system. Despite advancements in diagnostic methods, early detection remains challenging. Metabolomic analysis offers a promising, non-invasive approach to identify potential biomarkers for improved diagnostic precision.Cerebrospinal fluid (CSF) and serum samples from 24 healthy controls, 48 MS patients, and 20 Non-MS patients with a range of diverse conditions, including infections, autoimmune disorders, inflammatory issues, and other neurological disorders were collected during lumbar puncture. NMR analysis on a

Wasteosomes (or corpora amylacea) appear in the human brain during aging and in some neurodegenerative conditions. They entrap waste substances originated from different cell types and can be released from the brain to the cerebrospinal fluid (CSF). Their presence in the CSF was described using electron microscopy, PAS staining and immunofluorescence with natural IgMs and anti-glycogen synthase (GS) antibodies. However, the methods employed involved the use of a large sample volume for each staining, which hindered wasteosomes characterization. In the present work, we examined a new method to