Topic: JEDI-2P

Although dendritic signaling strongly influences sensory processing and learning, the connection between dendritic integration and somatic output is still not fully understood. Our aim is maping the origins of dendritic events during different aspects of visual learning.We used 3D two-photon laser scanning microscopy with acousto-optic deflectors to simultaneously record jRGECO1a (calcium) and JEDI-2p (voltage) signals in the apical dendrites of layer 5 pyramidal cells in the primary visual cortex of mice during visual discrimination. We examined mice with controlled water access participating

Newly developed voltage indicators require fast scanning methods for effective signal recordings. The flexibility of AO imaging allows us to measure in a three-dimensional volume not only at the somatic level, but also the dendrites. Their high scanning frequency makes AO microscopes suitable for dendritic signal processing and plasticity research. Soma dependent and independent dendritic activities are highly investigated areas, but the spatio-temporal limitations of electrophysiology and conventional one- or two-photon devices have serious constraints when it comes to GEVI imaging. Here we s