Topic: L-DOPA

L-DOPA-induced dyskinesia is a debilitating adverse effect of treating Parkinson’s disease with this drug. New therapeutic approaches that prevent or attenuate this side effect is clearly needed. Wistar adult male rats submitted to 6-hydroxydopamine-induced unilateral medial forebrain bundle lesions were treated with L-DOPA (oral or subcutaneous, 20 mg kg-1) once a day for 14 days. After this period, we tested if doxycycline (40 mg kg-1, intraperitoneal, a subantimicrobial dose) and COL-3 (50 and 100 nmol, intracerebroventricular) could reverse LID. In an additional experiment, doxycycline was

Dopamine plays a crucial role in the neural mechanisms underlying behavior, yet quantifying its impact through genetic modifications presents significant challenges, particularly due to the high-dimensional nature of the resulting data. In this study, we address these challenges by proposing a novel methodological framework using the Random Forest algorithm, tailored for robust multivariate analysis without the typical assumptions required in conventional statistical testing. Our experimental approach involved detailed video recordings of Drosophila melanogaster larvae, focusing on the effects