Topic: laser speckle contrast imaging

Aims: Resting-state functional imaging (RSFI) is increasingly used to understand how distinct brain networks modulate and respond to pain. As migraine, a common headache disorder, can be experimentally modeled by triggering cortical spreading depolarizations (CSD) in rodents, it is crucial to understand its impact on network connectivity to find imaging markers for experimental evaluation of headache and trigeminovascular activation. We used wide-field intrinsic optical-signal imaging (IOSI) to investigate the impact of CSDs on bihemispheric RSFI connectivity. Methods: C57BL6-ChR2mice underwen

Introduction: Neurovascular coupling (NVC) becomes impaired upon acute ischemic stroke (AIS). NVC dysfunction is thought to be linked to the evolution of spreading depolarization (SD), that causes vasoconstriction by augmenting the synthesis of 20-HETE. Here, we demonstrate the SD-related disruption of NVC during reperfusion, and block 20-HETE production to improve NVC.Methods: C57BL/6 mice (n=41) were anesthetized with isoflurane. Cerebral blood flow (CBF) variations were captured using laser speckle contrast imaging (LASCA) or laser-Doppler flowmetry. After 10 min baseline recording, transie

The mechanisms underlying neurodegeneration in multiple sclerosis (MS) remain incompletely understood. In this study, we aimed to investigate the role of vascular dysfunction in cortical neurodegeneration using a chronic cranial window model of experimental autoimmune encephalomyelitis (EAE) in mice.Following the establishment of chronic cranial window and induction of EAE with MOG peptides in C57BL6/J mice, we assessed cerebrovascular reactivity (CVR) using both laser speckle contrast imaging (LSCI) and optical signal imaging (IOSI) in awake animals, with stimuli including air puff to the whi