Lipidomics

Due to the complexity of CNS, studying the aging processes at the level of neural circuits and individually identified neurons is not an easy task in vertebrates, hence aging research heavily relies on invertebrate models. One such model is the great pond snail (Lymnaea stagnalis), which has been used extensively to study cognitive aging.We made the neuronal transcriptome assembly of Lymnaea and identified several evolutionarily conserved homolog sequences to genes involved in aging, age-related memory impairment, and neurodegenerative diseases of vertebrates including humans. Using young (3-4

A combination of genetic and environmental factors likely underlies most neurodegenerative diseases. Among environmental factors, metabolic disease is an emerging risk factor, but the mechanisms that confer risk are still poorly understood. We hypothesise that diet-induced obesity dysregulates lipid metabolism and promotes glial activation in a mouse model of Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD). In particular, we aim to study the role of altered lipid metabolism and neuroinflammation in response to short (12 weeks) and prolonged (14-16 months) high-fat diet (H

Our aim is to investigate the effect of choline, fish oil, and uridine-5'-monophosphate (UMP) supplementation on lipid profiles in the 5XFAD mouse model of Alzheimer's disease (AD) was to characterize cholesterol, glycerophospholipids (GPLs), sphingolipids (SLs), glycolipids (GLs), lysophospholipids (lyso GPLs) and lysophospholipid acyltransferases (lyso GPL O-ATs), and glycerophospholipid (GPL Os).Lipidomic analysis was performed on hippocampal brain tissue samples from 3-, 6- and 9-month-old 5XFAD mice and littermate controls using cutting-edge mass spectrometers with direct infusion (shotgu

FTY720 (fingolimod), a synthetic sphingosine 1-phosphate (S1P) analogue, is a drug employed for treating relapsing-remitting multiple sclerosis in diverse age groups. With the ability to cross the blood-brain barrier, FTY720 gradually accumulates within lipid-rich areas of the central nervous system (CNS) by integrating into phospholipid membranes. Consequently, FTY720 can enhance cell membrane fluidity, influencing glial cells and neuronal populations involved in behaviour regulation. This study investigated the impact of chronic FTY720 administration (3.3 μg/ml in ad libitum drinking water f

The neuronal plasma membrane is composed of versatile lipid molecules with distinct biophysical characteristics and functions. Among them, sphingomyelin (SM) influences the localization and functions of various proteins including GPI-anchored proteins. Therefore, elucidation of the mechanisms that regulate the SM amount is important for understanding the mechanisms that control neuronal morphology and information transmission. Reelin is a secreted protein that regulates neuronal morphology and dynamics. We previously found that the embryonic brain of Reelin-deficient mice shows aberrant lipid

Fatty acids have long been considered essential for brain development, however, the involvement of fatty acid synthesis in nervous system formation is unclear. Our previous approaches using the proteomics of the growth cone revealed enrichment of the proteins involved in fatty acids (ref. 1), such as fatty acid synthase or fatty acid-binding proteins. To analyze how fatty acids contribute to the neuronal network formation, we generated mice with knockout of GPSN2, an enzyme involved in fatty acid elongation and synthesis of very long-chain fatty acids (VLCFA), and investigated the impact on ne