Topic: middle cerebral artery occlusion

Stroke is the main cause of adult disability in developing countries. There is a need for identifying new signalling pathways involved in stroke pathogenesis to discover potential therapeutic strategies for stroke. We previously found that the E3 ubiquitin ligase APC/C-Cdh1 promotes neurogenesis, proliferation, and neuronal survival, which are essential events for brain repair after stroke. APC-Cdh1 also regulates protein levels of the dendrite stabilizer Rock2. Thus, the APC/C-Cdh1-Rock2 axis modulates dendrite elongation and synaptogenesis. However, whether Cdh1 plays a role in stroke remain

Acute ischemic stroke, also referred to as a cerebrovascular accident, occurs when localized tissue faces ischemia, leading to an inability to maintain physiological conditions or even resulting in death due to the obstruction or rupture of blood vessels in the brain. The current use of rt-PA for stroke medication has a limited time frame of 3-4.5 hours and is associated with neurotoxicity. Hence, there is an urgent need to explore alternative treatments for ischemic stroke. Cinchonidine, an alkaloid derived from the bark of cinchona, has previously demonstrated anti-inflammatory and antioxida

Clonal hematopoiesis, which refers to somatic mutations in hematopoietic stem cells (HSC), is recognized as a risk factor for stroke. Tet2 mutations exerted the greatest influence on complex cardiovascular endpoints in a study of incident strokes. The aim of this preclinical study was to explore if mutant HSC exhibit an aggressive phenotype, releasing pro-inflammatory cytokines and causing more damage to the penumbra area, heart, endothelial cells, and the blood-brain barrier (BBB). One-leg irradiated mice grafted into with WT or Tet2-/- (induced knockout) chimeric bone marrow were subjected t

Adult cerebral ischemia (stroke) is a neurological injury caused by the occlusion of cerebral blood vessels and it is one of the leading causes of long-term disability. Notably, an ischemic stroke can occur also during brain development with the early perinatal period being characterized by critical period windows when experience provides essential information for the proper maturation of sensory, motor, and cognitive functions. Despite the large amount of evidence on adult stroke, very little it is still known about the impact of a stroke occurring during perinatal ages in the developing brai

Neonatal ischemic stroke is a significant cause of mortality, severe developmental delays, and spastic cerebral palsy. In most cases, ischemic stroke occurs in the basin of the middle cerebral artery. Middle cerebral artery occlusion is the well-established model for stroke in adults. However, this method is mostly described in rat pups and is not well-established in neonatal mice.In this study, we compared a model of neonatal stroke (tfMCAO, transient filament middle cerebral artery occlusion) with neonatal hypoxic-ischemic brain injury (RVM, Rice-Vannucci model of unilateral carotid ligation

The dynamic role of neuroinflammation in diverse neurological disorders is still poorly characterized but could represent a promising avenue for therapeutic interventions. Here we aimed at characterizing brain-wide response of microglia to ischemic stroke in mouse. Microglia, the frontline responders to ischemic stroke, is known to exhibit diverse morphology and gene expression in response to diverse stimuli and depending on tissue context, yet how their activation signature propagates across the brain in response to local stroke has remained unknown. Here we established a whole-brain three-di