Topic: nanoparticle tracking analysis

The dynamics of biological systems depend on complex cell-cell interactions. Extracellular vesicles (EVs) are nanosized vehicles of intercellular communication which shuttle several molecules from cell to cell, thus influencing relevant biological processes. Previous literature based on EV isolation from cell culture medium highlighted how EVs in the peripheral nervous system could be important mediators in the molecular dialogue between neurons and Schwann cells. Here, we aim to extract EVs directly from peripheral nerve tissue, as this would give the unique opportunity to characterize EVs fr

Extracellular vesicles (EVs) are membrane-enveloped nanoparticles released into the extracellular space by almost all cell types. They have been shown to spread α-synuclein (αSyn) – the main pathological hallmark of Parkinson´s disease (PD) – and miRNAs that can influence the expression of genes associated with PD progression. On this basis, they are becoming interesting as diagnostic biomarkers. So far, however, data from patients are heterogeneous and inconsistent. Therefore, we aim to use a cellular model that allows us to investigate the role of EVs in PD pathology on a homogeneous genetic