Topic: neural organoids

The flexibility and accessibility of induced pluripotent stem cell (iPSC) technology has allowed complex human biology to be reproduced in vitro at high throughput scales. Indeed, rapid advances in stem cell technology have led to widespread adoption for the development of in vitro models of neuron electrophysiology to be used in screening applications in drug discovery and safety. Furthermore, advanced cell preparations, such as spheroids or organoids, are under intense investigation with aims toward establishing mature physiologically relevant phenotypes in vitro. The objective of this work

Koolen-de Vries syndrome (KdVS), also known as 17q21.31 microdeletion syndrome, is a rare neurodevelopmental disorder characterized by developmental delay, intellectual disability, and congenital malformations in multiple organ systems, including the brain. KdVS is caused by haploinsufficiency of KANSL1, a gene encoding a protein involved in chromatin remodeling during interphase and microtubule stabilisation during mitosis. KANSL1 is widely expressed in early development, but its role in early brain formation is not characterized. Using patient and genetically-edited induced pluripotent stem

Human induced pluripotent stem cell (hiPSC)-derived neural models have emerged as invaluable tools for studying neurological disorders, such as epilepsy, Alzheimer’s, and Parkinson’s disease.Real-time, label-free measurement of electrical activity in self-organizing in vitro cellular models provides critical insight into the complexity of their neuronal networks. High-density microelectrode arrays (HD-MEAs) enable non-invasive electrophysiological recordings from various electrogenic samples, including iPSC-derived neurons, retinal explants, brain slices, and neural organoids.In this study, we