Topic: novel object recognition test

Alzheimer’s disease (AD) is a progressive age-associated brain disorder in which deficits in episodic memory function are among the first cognitive symptoms observed. Clinical findings indicate that AD patients are more susceptible to anesthesia-induced postoperative cognitive dysfunction, which is in line with the well-known fact that general anesthesia is more detrimental in the two extreme age groups, neonates/infants and the elderly. Alfaxalone (ALX) is the synthetic analog of allopregnanolone. It is currently in use only in veterinary practice as a fast-acting anesthetic with a high thera

In this work, we want to dissect the specific and sex-dependent role of CB1 signalling in different cell types and its impact on hippocampal functions such as navigation or memory. A specific Cre-mediated CB1 deletion on neurons (excitatory and inhibitory), astrocytes, or interneurons in CB1-flox males and females was performed. Viral vectors (AAV-Cre) were injected bilaterally into the hippocampus of these mice. The Barnes maze was used for learning, memory, navigation and the Novel Object Recognition Test (NORT) for recognition memory. Anxiety-like behavioural protocols were also performed b

Trimethyltin (TMT)-induced neurodegeneration leads to molecular and behavioral changes similar to those of Alzheimer's disease (AD). Continuous theta burst stimulation (cTBS) has shown promise in psychiatric and neurological disorders but remains largely unexplored in AD models. For this reason, a well-characterized animal model of TMT-induced hippocampal neurodegeneration was used to investigate, for the first time, the effects of cTBS on learning/memory and behavioral disturbances.Adult male Wistar rats were divided into four experimental groups: intact control, TMT (8mg/kg, ip) and TMT trea

Age-related declines in sensory, motor, and cognitive abilities are a major risk factor for neurodegenerative diseases. Dietary restriction (DR) can slow or postpone these changes, improving memory, learning, and cognitive abilities, but may not always yield positive results. In this study, we examined the impact of 30% DR (70% of ad libitum daily intake) during adolescence on the short-term (STM) and long-term (LTM) memory performance in 6- and 18-month-old female Wistar rats. The effects of DR were examined in three onsets and durations: DR1 (28th to 35th day of life), DR2 (35th to 42nd day

Calorie restriction (CR) is recognized for its potential to delay age-related changes; however, its effectiveness can vary based on factors such as duration, age, and sex. Implementing CR later in life may not only diminish its protective potential but could also have detrimental effects. Herein, we aimed to investigate the effect of CR on short-term (STM) and long-term (STM) memory and explore the potential role of the mTOR signaling pathway as an underlying molecular mechanism. Female Wistar rats of different ages (adult, middle-aged, and old) were exposed to CR (60% of ad libitum (AL)) to s

Sleep benefits memory consolidation, especially the first hours following learning, as shown in humans and rodents. However, the effect of sex on both memory performance and its dependency on sleep remains poorly investigated. To investigate the interaction between sex and sleep on memory consolidation, we employed the Novel Object Recognition Test (NORT) in rodents, a task reliant on post-learning sleep for consolidation. Six-month-old male and female mice (n=10/group) were trained with two objects, followed by either undisturbed sleep (control group) or 5 hours of sleep deprivation (SD grou

Major neurocognitive disorder (NCD) affects over 55 million people worldwide and is characterized by cognitive impairment (CI). This study aimed to develop a non-invasive diagnostic test for CI based upon retinal thickness measurements explored in a mouse model. Discrimination indices and retinal layer thickness of healthy C57BL/6J mice were quantified through a novel object recognition test (NORT) and ocular coherence tomography (OCT), respectively. Based on criteria from the Diagnostic and statistical manual of mental disorders 5th ed. (DSM-V), a diagnostic test was generated by transforming

Depression, affecting 280 million people worldwide, is marked by a significant treatment gap. Current therapies with delayed onset of action fail to alleviate the symptoms in almost half of patients, often neglecting the cognitive deficits. This highlights the critical need for fast-acting antidepressants with memory-enhancing properties. HBK-15, a multimodal compound, previously demonstrated antidepressant-, anxiolytic-like and antiamnesic effects in rodents. Our study aimed to explore HBK-15's potential in reversing stress-induced behavioral abnormalities and elucidating its underlying mecha