Topic: optogenetic actuators

Biological neural networks show an inherent structure and connections that are reproduced across individuals. It is still largely unknown how the structure of such networks is correlated with their observed functional dynamics and computational output. Detailed investigation of network structure and its functional implications is challenging. For instance, in vivo studies do not allow for isolated analysis of network motifs. As basic elements of neuronal systems, these motifs need careful consideration. In contrast, the main obstacle in vitro is to create defined network structures down to the

The locus coeruleus (LC) noradrenergic (NE) system is involved in a plethora of physiological and pathophysiological processes. Refining our understanding of LC function largely relies on selective transgene expression in molecularly defined cells, allowing targeted manipulation and readout of noradrenergic neurons. Here, we performed a side-by-side comparison of the most commonly used strategies to genetically access the LC, including different cre driver lines and promoter-mediated transgene expression. We report differences between these strategies in terms of transgene expression efficacy