ePoster · Histology
New method to characterize wasteosomes (corpora amylacea) from the human intraventricular cerebrospinal fluid
Marta Riba, Raquel Alsina, Clara Romera, Jaume del Valle, Jordi Vilaplana, Laura Molina-Porcel, Carme Pelegrí · FENS Forum 2024
Wasteosomes (or corpora amylacea) appear in the human brain during aging and in some neurodegenerative conditions. They entrap waste substances originated from different cell types and can be released from the brain to the cerebrospinal fluid (CSF). Their presence in the CSF was described using electron microscopy, PAS staining and immunofluorescence with natural IgMs and anti-glycogen synthase (GS) antibodies. However, the methods employed involved the use of a large sample volume for each staining, which hindered wasteosomes characterization. In the present work, we examined a new method to