Topic: qPCR

Danio rerio (zebrafish) is a model for studying neurophysiological development and gene mutation consequences. Zebrafish possesses orthologs of human NMDA receptor subunit genes, including grin2Aa and grin2Ab for GRIN2A. The grin2Ab knockout strain created via CRISPR-Cas9, and the grin2Aa knockout were investigated for spatial and temporal gene expression and swimming behavior. Whole-mount in situ hybridization (WISH) was performed on wild-type (WT) zebrafish larvae aged 1, 3, and 5 days post fertilization (dpf). The results indicated minimal expression of both genes at 1 dpf. By 3 dpf, grin2A

Aims: The identification of peripheral biomarkers of brain dysfunctions is essential to aid diagnosis and facilitate patient stratification. MicroRNAs are excellent candidate molecules to this purpose, as they can be expressed in the brain with regional and functional specificity, and they are highly stable in blood serum, unlike other RNA transcripts. Mir-34a is expressed with high specificity in a subpopulation of GABAergic neurons within the mouse Dorsal Raphe Nuclei (DRN), where it regulates GABAergic transmission in response to aversive stimuli. However, a direct connection between the br

Background: We study dopaminergic neurons with specific emphasis on Parkinson’s disease. Our work involves implementing procedures for examining in vitro mouse primary dopaminergic neurons as well as dopaminergic neurons derived from human stem cells. Here we want to establish a continuous cell line model for fast and cost-effective production of human dopaminergic-like cells to be used in our studies. Aims: Differentiate SH-SY5Y cells into dopaminergic-like cells using Retinoic Acid. Optimize the differentiation protocol for highest yield of TH positive cells. Methods: Retinoic Acid is used t