Topic: rotenone

Parkinson’s disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra (SN) pars compacta, leading to motor and cognitive impairments. The lack of a cure for this disease highlights the urgency in finding drugs that can modify its course. This emphasizes the importance of creating preclinical models that mimic major aspects of the disease. Rotenone, a natural pesticide that inhibits the mitochondrial complex I, has been shown to induce inflammation and selectively destroys nigrostriatal dopaminergic cells and thus presents itself as a

The goal of this work is to analyze the mitochondrial changes that Rotenone (Rot) treatment can induce in primary cortical neurons that could contribute to the schizophrenia-like phenotype animal model, previously demonstrated by our research group. For this, we previously determined the Rotenone (Rot) concentration in the cortex (1.325nM) from our animal model with a schizophrenia-like phenotype. Then, we treated the primary cortical neurons with Rot 1.325nM for 24h and investigated: the Rot cellular viability treatment by MTT and CytotoxGlo assay; CI enzymatic activity; oxygen consumption ra

We aimed to develop and standardize a diet and rotenone-based animal model of Parkinson's disease. To achieve this, 21 days old male C57BL/6 mice were fed on a high-fat diet [HFD] (60%kcal) for 17 weeks; starting from week 11, animals were orally gavaged daily with 10mg/kg rotenone dose till the end of the 17th week. Further, they were subjected to behavioral tests (Rota-rod, Y maze, and fecal pellet output test). Fecal pellets were collected for 16S rRNA sequencing and animals were subsequently euthanized. Our data revealed that rotenone and its combination with HFD exhibited loss of motor fu

Parkinson's disease (PD) is progressive neurodegenerative disease characterized by the selective loss of dopaminergic neurons and accumulation of α-synuclein in the substantia nigra. Neurotoxins, such as rotenone and 6-hydroxidopamine are widely used to induce in vitro model of PD. These neurotoxins induce mitochondrial dysfunction and increase oxidative stress, leading to neuronal degeneration and cell death by apoptosis. Dehydroepiandrosterone (DHEA) and its sulfate (DHEAS), along with brain-derived neurotrophic factor (BDNF) are involved in neuroprotection. Levels of both DHEA(S) and BDNF d

Cytokine receptor-like factor 3 (CRLF3) is an phylogenetically ancient class 1 cytokine receptor present in all major eumetazoan taxa ranging from cnidarians to mammals. In humans, CRLF3 is expressed in various tissues and has been associated with proliferation, differentiation, cell survival and some diseases. Our studies on insect neurons and human iPSC-derived neurons demonstrated that CRLF3 (whose insect-endogenous ligand is still unidentified) can be activated by human erythropoietin (EPO) and its non-erythropoietic splice variant EV-3. Under apoptogenic conditions, CRLF3 initiates pro-su