Topic: Row-Column Addressing

3D ultrasound localization microscopy (3D-ULM) allows in vivo quantification of blood vessel velocities in the mouse brain using matrix arrays, but it requires complex electronics, and the field of view remains limited [1]. Here, we investigate how low channel count Row-Column Addressing (RCA) probes allow 3D-ULM to be performed in the whole mouse brain non-invasively and in the visual cortex of a non-human primate after craniotomy.An IcoPrime 4D-RCA probe (80+80 elements, 15 MHz, pitch 0.11 mm, Iconeus) was driven by an Iconeus One scanner. For the mouse experiment, the probe was mounted on a

Functional ultrasound (fUS) imaging has recently allowed 3D functional imaging in awake mice using motorized linear or matrix arrays (Brunner et al., 2020; Bertolo et al., 2021). However, the tradeoff between the field of view and temporal resolution introduced by motorized scanning hinders acquiring whole-brain resting state Functional Connectivity (rsFC), whereas the limited sensitivity of matrix arrays prevents transcranial imaging in mice. Here we propose a new hybrid approach dedicated to 3D rsFC in the whole mouse brain with intact skull.A newly developed 15 MHz multiarray probe (IcoPrim