Topic: Ryanodine receptors

Calcium, an important secondary messenger, lies at the hub of multiple signaling pathways. The increase in cytosolic calcium contributed by different calcium-permeable channels can create transient high-calcium microenvironments and activate distinct signaling cascades in neurons. The spatio-temporal calcium changes, and calcium-mediated signaling pathways act as critical regulators of synaptic plasticity. Here, we have studied the regulation of activity-mediated protein synthesis by calcium response generated on NMDAR or mGluR stimulation in rat primary cortical neurons. Using calcium imaging

Understanding the mechanisms regulating Ca2+ concentrations in the presynapse is crucial for addressing synaptic function. Most factors contributing to short-term plasticity, serving as a historical record of past synaptic transmission, are located in the presynaptic terminal. We investigated the influence of calcium exchange in different spatial compartments and the dynamics of calcium concentration in the cytosol and the endoplasmic reticulum (ER). Thus, we unveiled the impact of calcium in the ER on the dynamics of cytosolic calcium concentration in response to action potentials. We establi

Calcium released from intracellular stores is critical for controlling cellular processes such as secretion, synaptic transmission, and gene expression. Ryanodine receptors (RyRs) are Ca2+-permeable channels found in the endoplasmic reticulum. Currently, the relationship between Ca2+-induced Ca2+release (CICR) via RyRs and Ca2+-dependent synaptic transmission is unclear. Here, we investigated the role and potential mechanisms of RyR-mediated Ca2+ release at excitatory synapses. Experiments were performed on mouse hippocampal autaptic neurons (hANs) and adrenal chromaffin cells (CCs). The analy

Lactate is an important metabolite produced by astrocytes and delivered to neurons to support their energy needs in a process known as the astrocyte-neuron lactate shuttle. Emerging research during the past decade has unveiled a role of lactate as a signal capable of orchestrating critical functions ranging from neuroprotection to memory. In previous work, we found that lactate enhanced the amplitude and decay time of NMDA receptor currents (INMDAR) evoked by brief applications of glutamate and glycine. These effects were not observed when neurons were exposed to lactate receptor (HCA1) agonis