Topic: sciatic nerve crush injury

Schwann cells (SCs) are the principal glia of the peripheral nervous system and are in close contact with axons to ensure a fast propagation of electrical impulses. After nerve injury, myelinating SCs distal to the injury site dedifferentiate into pro-regenerative repair SCs. In a later phase after injury, repair SCs must redifferentiate into myelinating cells to provide a fully functional regenerated nerve. Meyer zu Reckendorf et al. recently showed the important role of metabolic adaptation in SC reprogramming after injury in an ex vivo nerve lesion model. In particular, lipid metabolism dow

In this study, we aimed to explore methylcobalamin (MeCbl), a common treatment for peripheral nerve injuries, and its impact on macrophage phenotypes (inflammatory or anti-inflammatory) at nerve injury sites and the underlying mechanism. Using a sciatic nerve crush injury model in 6-week-old Wistar rats, we administered either saline (control group) or MeCbl (MeCbl group) via osmotic pumps for sustained systemic delivery. We assessed macrophage phenotypes in transverse sections of the sciatic nerve up to 14 days post-surgery using fluorescence immunostaining. In vitro, we induced inflammatory