Topic: scRNAseq

Single-cell methodologies have improved our understanding of cellular diversity within complex tissues such as the mammalian neocortex. However, despite the unprecedented granularity, sampling of minority yet diverse cell populations remains challenging. Here, we show that many subtypes of cortical inhibitory γ-aminobutyric acid-containing (GABAergic) neurons are poorly represented in current atlases, with most datasets limited to adult stages and not recapitulating cell-state transitions of these rare cell types during development. We address this gap by optimizing experimental techniques and

Abnormal reward and threat processing are key players in psychiatric conditions such as eating disorders, addiction or depression. These functions are strongly regulated by the nucleus accumbens shell (NAcSh) which integrates emotional signals to regulate motor output. Historically the NAcSh was thought to consist of 2 pathways: dopamine 1 and 2 receptor-expressing striatal projection neurons (D1, D2-SPNs) which promoted reward and aversive behavior, respectively. Yet recent work has revealed that both cell types can promote either reward or aversion, depending on the NAcSh subregion or input/