Topic: Sigma-1 receptors

Background: Microglia activation is an early response in ischemic brain injury, which initiates neuroinflammation. Microglia express Sigma-1 receptors (Sig-1R) at the mitochondria-associated membranes (MAM) of the endoplasmic reticulum(ER), which have been implicated in cellular stress responses. Here we set out to evaluate whether the Sig-1R agonist N,N-dimethyltryptamine (DMT) modulates microglia activation. Materials and methods: Isolated microglia cultures were prepared from the cortex of neonatal Sprague Dawley rats. On day 6, the cells were treated with lipopolysaccharide (LPS;20ng/ml) o

Lipid rafts are liquid-ordered domains where specific enzymes and receptors are located. These membrane platforms play crucial roles in a variety of signaling pathways. Thus, alterations in the lipid environment, such as those elicited by oxidative stress, can lead to important functional disruptions in membrane proteins. Cell membrane microarrays has emerged as a powerful methodology for the study of both lipids and membrane proteins, as they preserved native protein structure and lipid environment. Based on this technology, we developed membrane microarrays with different lipid raft preparat