Topic: single-cell measurements

Laura Graus explains a multi-omics workflow that combines B-cell receptor repertoire analysis with phenotypic profiling using dCODE Klickmer technology. The seminar examines how the pipeline characterizes B-cell responses after vaccination and infection, including the function and dynamics of memory B-cell reactivation. Its application to vaccination studies illustrates how single-cell measurements can inform HIV-1 vaccine design.

Single-cell measurements of mRNA copy numbers inform our understanding of stochastic gene expression, but these measurements coarse-grain over the individual copies of the gene, where transcription and its regulation take place stochastically. We recently combined single-molecule quantification of mRNA and gene loci to measure the transcriptional activity of an endogenous gene in individual Escherichia coli bacteria. When interpreted using a theoretical model for mRNA dynamics, the single-cell data allowed us to obtain the probabilistic rates of promoter switching, transcription initiation and