BDNF/TrkB signalling, in cooperation with muscarinic signalling, retrogradely regulates PKA pathway to phosphorylate SNAP-25 and Synapsin-1 at the NMJ
Aleksandra Polishchuk, Víctor Cilleros-Mañé, Marta Balanyà-Segura, Laia Just-Borràs, Carolina Silvera Simón, Marta Tomàs, Meryem Jami El Hirchi, Erica Hurtado, Josep Tomàs, María Angel Lanuza · FENS Forum 2024
PKA, influenced by retrograde signalling from muscle contraction, enhances neurotransmission at the neuromuscular junction (NMJ). This occurs through the phosphorylation of key molecules in synaptic vesicle exocytosis, SNAP-25 and Synapsin-1. The BDNF/TrkB pathway, augmented by muscle contraction, is a known regulator of retrograde control in ACh exocytosis at the NMJ. This modulation, including muscarinic receptors M1 and M2 (mAChRs) linked with both PKA and BDNF/TrkB signalling, has not been studied until now. Here, we investigate how synaptic activity and muscle contraction regulate PKA sub