Topic: Spatial proteomics

Early Alzheimer’s disease (AD) stages (that start even decades before clinical disease onset) are characterized by hippocampal dysfunction induced by amyloid-β oligomers (oAβ). Indeed, we have recently characterized hippocampal-dependent memory encoding and retrieval deficits and synaptic plasticity impairments in a non-transgenic mice model of early AD amyloidosis. Some protein changes underlying those deficits have been previously studied, but the spatial distribution within the hippocampus, and the possible sex dimorphism, has not been investigated before. Since each hippocampal part has cl

Neurons in the centromedial amygdala (CeM) are activated during fear memory formation. We designed a new viral vector, AAV9-pfos-hM4Di:mCherry:PEST expressing Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) under a c-fos promoter, enabling the specific manipulation of activated neurons. We use this viral vector to temporarily inactivate the neurons involved during cued-fear learning in the CeM and to uncover their molecular profile.Male and female wildtype C57BL/6J mice are injected with the viral vector in the CeM, exposing one group to the shocks paired with the tones (f