Topic: transient middle cerebral artery occlusion

Stroke is one of the major reasons for death and disability. Insufficient blood flow in stroke interferes the brain from proper oxygenation, leading to the stabilization of hypoxia-inducible factors (HIFs). To determine the role of HIFs in stroke, particularly HIF-2α, an in vivo model called tMCAO (transient middle cerebral artery occlusion) and an in vitromodel with human SH-SY5Y cells was used. On both, Hif-2α deficient and wild type mice, tMCAO was conducted for two hours. In addition to RNA and protein samples, tissue for immunofluorescence microscopy was collected of ischemic and control

Microglia are crucial players in brain injury, orchestrating inflammatory responses, scavenging cellular debris, and promoting plasticity for functional recovery. To perform these functions, microglia undergo specific metabolic adaptations. Ex vivo evidence shows that, when subjected to glucose depletion, microglia are able to maintain their motility and surveillance, thanks to their high metabolic flexibility. However, the energetic sources sustaining their flexibility in vivo remain unclear. Ischemic stroke is characterized by a sudden drop in nutrients and oxygen supply, while extracellular