Topic: TRPA1

Alzheimer’s disease (AD) is characterized by a long asymptomatic phase associated with the toxic accumulation of oligomeric form of amyloid-beta peptide (Aβo). One of the first event reported in this phase seems to be a specific hippocampal hyperactivity in both humans and AD mouse model. We highlighted that this early hyperactivity starts with an abnormal increase of calcium activity in astrocytes which affects neighbouring neurons in the hippocampus. We identified an astrocytic calcium channel, transient receptor potential ankyrin-1 (TRPA1), implicated in the Aβo-induced hippocampal hyperact

Introduction: Transient receptor potential ankyrin 1 (TRPA1) is a non-selective cation channel expressed in the urocortin 1 (UCN1) positive neurons of the centrally projecting Edinger-Westphal nucleus (EWcp) having modulatory impact on stress adaptation. As post-traumatic stress disorder (PTSD) is triggered by disturbed stress adaptation, we hypothesized the regulatory role of EWcp/UCN1/Trpa1 neurons in PTSD.Methods: Male TRPA1 wild-type (WT) and knockout (KO) mice were exposed to two different models of PTSD. In single prolonged stress (SPS) paradigm, time spent immobility was measured during

Dry eye disease (DED) involves neurosensory abnormalities and inflammation. The aim of this study was to elucidate the role of transient receptor potential ankyrin-1 (TRPA1), a highly expressed corneal nociceptor, in neurogenic inflammation associated with persistent DED.Multi-unit extracellular recording of naive mice ciliary nerve was monitored with/without TRPA1 antagonist (HC030031), to determine which corneal nerve fiber is inhibited by this antagonist. DED was induced in mice by removing the extraorbital lacrimal gland and Harderian gland. Mice underwent topical treatment with HC030031 o

Mutations within ion channels can cause functional alterations, including either loss or gain of function. Specific activation of the TRPA1 channel has been shown to be sufficient to trigger pain in humans [Heber et al, 2019]. The dbSNP database records five missense single nucleotide polymorphisms (SNPs) within the TRPA1 gene - R3C, R58T, E179K, K186N, and H1018R, with the second most frequent variant exceeding 10% in the general population. This study aimed to compare these genetic variants concerning their impact on established modes of activation in contrast to the most common form of TRPA

Transient receptor potential ankyrin 1 (TRPA1) plays a pivotal role in migraine pathogenesis via regulating cortical spreading depression, neuroinflammation, hyperalgesia and trigeminovascular system activation and sensitization. However, whether TRPA1 could affect photophobia, a symptom that affects ~80% migraineurs, is unknown. In this study, we examined whether TRPA1 activation triggers photophobia, and displays a gender-specific difference in photophobia behavior. C57BL/6J mice were used to assess mouse behavior in both dark and light compartments of a chamber. The photophobia behavior was

Scientifica PatchScope Pro system is an integrated electrophysiology rig incorporating an inverted phase-contrast fluorescence microscope, motorised XY stage and PatchStar micromanipulators, suitable for patch-clamp recording. In contrast to other patch-clamp systems, this microscope has a small footprint to maximise use of lab space. The low-noise electrical components are designed specifically for patch-clamp recordings. Fluorescence capability allows identification of cells of interest (e.g. using fluorescent markers or calcium-imaging) and motorisation of the stage means that XY co-ordinat

In the pursuit of restoring vision for patients having lost the eye-brain connection, ultrasound stimulation is a new interesting therapeutic approach at the cortical level. In particular, Sonogenetic therapy has been explored as a mean to increase the sensitivity of cells to ultrasound by expressing a mechanosensitive ion channel in the membranes of cells of interest in order to modulate their activity.In order to optimize sonogenetics for vision restoration, we first focused on exploring the properties of different mechano-sensitive ion channels, the key parameters being the spatial and temp

Statins represent a class of drugs commonly prescribed for lowering cholesterol levels, thereby reducing the risk of atherosclerotic cardiovascular disease. While accumulating evidence indicates an increased risk of peripheral neuropathy with prolonged statin use in humans, in vivo animal studies revealed an anti-nociceptive effect of these drugs without fully elucidating the underlying mechanisms. Transient receptor potential (TRP) ion channels represent a major class of receptors that are expressed in the peripheral nervous system and are involved in pain signaling. Among these channels, TRP

Dorsal root ganglion (DRG) neurons are quintessential for peripheral pain transduction, a process mediated, among others, by certain members of the transient receptor potential (TRP) superfamily of ion channels. Among those, TRPA1, TRPV1, TRPM3 and TRPM8 have been linked to chronic pain and neuropathies. Tumor suppressor candidate 5 (Tusc5) plays a role in adipocyte function, but is also expressed in all major classes of DRG neurons. Tusc5 function in DRGs has not been investigated. We conducted Fura-2 calcium imaging and patch clamp experiments in cultured DRG neurons from knock-in Tusc5eGFP

The molecular mechanism of the antimalarial artemisinin and its derivatives is not yet fully understood, although these compounds are successfully used against Plasmodium protists. Other biological effects (anti-cancer or antinociceptive effects) are also possible following administration of artemisinin. Considering that Transient Receptor Potential (TRP) ion channels are key signalling molecules in many cancer and pain pathways, the aim of this study was to test whether members of this family are among the molecular targets of artemisinin. The effect of artemisinin on wild type or mutant TRP