Topic: TRPM8

Neuropathic pain (NeuP) is a global health problem, with clinical manifestations including spontaneous (non-evoked) chronic pain (i.e. burning sensation) and altered somatosensory perception. To date, we still lack a detailed understanding of the molecular mechanisms driving NeuP. In preclinical mouse studies of NeuP, the main behaviour endpoints often fail to reflect typically observed patient symptoms, and the routine use of male subjects across diverse age ranges likely masks potential age- and sex-dependent variations. We aim to investigate the impact of age and sex on pain-related behavio

The sensation of cooling appears to depend largely on the transient receptor potential channel TRPM8. However, the detection of noxious cold is still controversial. Many targets have been proposed for the transduction of cold into an electrical signal. So far, there is no experimental human model that allows the application of potential antagonists at the same site as the cold. This is further complicated by the fact that the site of cold transduction is unclear.We have developed a human pain model based on intradermal injection of cooled extracellular solution into the volar forearm. This is

Scientifica PatchScope Pro system is an integrated electrophysiology rig incorporating an inverted phase-contrast fluorescence microscope, motorised XY stage and PatchStar micromanipulators, suitable for patch-clamp recording. In contrast to other patch-clamp systems, this microscope has a small footprint to maximise use of lab space. The low-noise electrical components are designed specifically for patch-clamp recordings. Fluorescence capability allows identification of cells of interest (e.g. using fluorescent markers or calcium-imaging) and motorisation of the stage means that XY co-ordinat

Dorsal root ganglion (DRG) neurons are quintessential for peripheral pain transduction, a process mediated, among others, by certain members of the transient receptor potential (TRP) superfamily of ion channels. Among those, TRPA1, TRPV1, TRPM3 and TRPM8 have been linked to chronic pain and neuropathies. Tumor suppressor candidate 5 (Tusc5) plays a role in adipocyte function, but is also expressed in all major classes of DRG neurons. Tusc5 function in DRGs has not been investigated. We conducted Fura-2 calcium imaging and patch clamp experiments in cultured DRG neurons from knock-in Tusc5eGFP

The Transient receptor Receptor potential Potential melastatin Melastatin family member subtype 8 (TRPM8) receptor-channel is involved in mammalian innocuous cold perception and is also known to mediate anti-inflammatory effects, reversing nociceptive hypersensitivity and reducing chronic pain. Besides the well-characterized effects of prostacyclin (PGI2) in the vasculature, there is a general recognition that PGI2 is also involved in nociception, as it induces pain sensitivity and increase inflammatory responses. These biological effects are mediated by the prostacyclin receptor (IP-R) expres

The transient receptor potential cation channel subfamily member 8 (TRPM8) is a cold-activated receptor whose expression in the peripheral nervous system supports the detection of environmental cold temperatures. Surprisingly, TRMP8 is also expressed in the lateral septum (LS), a basal ganglia region, even though the brain temperature varies little. The LS is an important hub for the regulation of motivated behaviors, integrating cortical information and controlling the activity of subcortical areas. We hypothesized that TRPM8 neurons from LS (LSTRPM8 neurons) are part of a volitional thermore