Topic: Vincristine

Vincristine (VCR), an anticancer drug, frequently causes chemotherapy-induced peripheral neuropathy (CIPN). We have reported that macrophages (Mφ)-derived HMGB1 is involved in CIPN induced by paclitaxel in mice, whereas the mechanisms for VCR-induced CIPN (VIPN) remain unclear. We thus examined whether VIPN involves HMGB1 derived from Mφ or Schwann cells in mice. VCR at 0.2 mg/kg was administrated i.p. to mice daily for 10 days with a 2-day break, and nociceptive threshold at the hindpaw was evaluated by von Frey test. An anti-HMGB1-neutralizing antibody (HMGB1-Ab), soluble thrombomodulin (TMα

Peripheral neuropathy is characterized by damage to the peripheral nerves, leading to symptoms such as numbness, tingling, and pain in the extremities. While peripheral neuropathy can be caused by several factors, a common cause is toxicity from chemotherapeutic drugs.We studied chemotherapy-induced peripheral neuropathy (CIPN) using the OrganoPlate, a microfluidic cell culture platform that allows culture of 40 chips in parallel. Human iPSC-derived peripheral neurons were embedded in extracellular matrix gel and seeded into the OrganoPlate, after which they extended their axons into an adjace

Microphysiological system (MPS) is an in vitro culture technology that reproduces the physiological microenvironment and functionality of humans, and is expected to be applied for evaluating drug efficiency/toxicity. In this study, we developed a MPS for structured culture of human iPSC-derived neurons, then predict drug-induced neurotoxicity by morphological analysis.COP (Cyclo olefin polymer), which has excellent observability and low drug adsorption, is used as the material of the MPS. Successful culture of human iPSC-derived sensory neurons and human iPSC-derived cortical neurons with sepa