Topic: widefield imaging

Understanding how cortical circuits generate complex behavior requires investigating the cell types that comprise them. Here, much effort has been focused on inhibitory neuron types but the functional roles of distinct classes of excitatory pyramidal neurons (PyNs) are less well un-derstood. We, therefore, used widefield imaging to measure the cortex-wide activity of distinct PyN types and investigated their functional role in mice that performed an auditory decision-making task. We used two mouse lines, expressing the calcium indicator GCaMP6s in two major PyN types: FezF2 for pyramidal-tract