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ePoster
EXTRACELLULAR MATRIX TUNES IMMUNOGLOBULIN DYNAMICS THROUGHOUT THE HIPPOCAMPUS
Ivo Calaresuand 4 co-authors
Interdisciplinary Institute for Neuroscience
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS03-08AM-365
Presentation
Date TBA
Board: PS03-08AM-365
Event Information
Poster Board
PS03-08AM-365
Poster
View posterAbstract
Effective neuronal communication and network function rely on the coordinated diffusion of molecules across varying distances within extracellular spaces (ECS). Our comprehension of spatial and temporal dynamics underlying these processes is critical for understanding physiological and pathological processes. Recent studies emphasize the extracellular matrix (ECM) as a complex and dynamic barrier that regulates the mobility of circulating molecules. We propose here a framework of advanced imaging techniques to address diffusion processes from the macro to the nanoscale in living brain slices. First, we exemplify brain ECS heterogeneity studying the fine-tuned rheology of the developing hippocampus. Transcriptomic screening of ECM protein genes led us to identify area-specific differences. By adenoviral transgene delivery in organotypic slices we modified ECM in order to abolish rheological disparities between hippocampal CA1 and CA3 subfields. Single-particle tracking (SPT) upon ECM manipulation confirmed the possibility to locally reduce macromolecular diffusion. Using multi electrode array, we performed extracellular field potential recordings on organotypic hippocampal slices exposed to pathological immunoglobulins. Through hindered diffusion, ECM manipulation successfully mitigated area-specific burden resulting from pathological macromolecules exposure.
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