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ePoster
EXTRACELLULAR VESICLES ISOLATED FROM THE SERUM OF WOMEN DIAGNOSED WITH MAJOR DEPRESSIVE DISORDER ALTER KEY PROCESSES FOR HUMAN MICROGLIA
Valeria Flores Torresand 1 co-author
Programa de Maestría en Ciencias Biológicas
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-674
Presentation
Date TBA
Board: PS06-09PM-674
Event Information
Poster Board
PS06-09PM-674
Poster
View posterAbstract
Major Depressive Disorder (MDD) is associated with inflammatory processes, in which microglia play a crucial role. Growing evidence suggests that soluble factors and extracellular vesicles, such as exosomes, may contribute to the pathophysiology of MDD. In this study, we present the standardization of exosome isolation and characterization from the serum of women diagnosed with MDD.
Exosome isolation was performed using size exclusion chromatography. The obtained fractions were evaluated by absorbance at 280 nm to identify fractions with low free protein content. Characterization was performed by slot blot analysis, detecting signals for the exosomal markers CD9, CD63, and Alix, and confirming the absence of Golgi contamination by the lack of GM130 signal. The results showed that fractions 2–4 were enriched in exosomes with minimal protein contamination. Quantification using a CD9-specific ELISA confirmed the presence of exosomes in the samples, with particle estimates ranging from 10⁸ to 10¹⁴, which is sufficient for cellular assays.
Additionally, in vitro assays for cell viability, cell migration, and exosome uptake were standardized. Overall, these preliminary data establish a methodological basis for investigating the impact of exosomes derived from MDDD patients on microglial function, anticipating changes in their effector functions and their potential contribution to neuroinflammatory processes associated with MDD.
Exosome isolation was performed using size exclusion chromatography. The obtained fractions were evaluated by absorbance at 280 nm to identify fractions with low free protein content. Characterization was performed by slot blot analysis, detecting signals for the exosomal markers CD9, CD63, and Alix, and confirming the absence of Golgi contamination by the lack of GM130 signal. The results showed that fractions 2–4 were enriched in exosomes with minimal protein contamination. Quantification using a CD9-specific ELISA confirmed the presence of exosomes in the samples, with particle estimates ranging from 10⁸ to 10¹⁴, which is sufficient for cellular assays.
Additionally, in vitro assays for cell viability, cell migration, and exosome uptake were standardized. Overall, these preliminary data establish a methodological basis for investigating the impact of exosomes derived from MDDD patients on microglial function, anticipating changes in their effector functions and their potential contribution to neuroinflammatory processes associated with MDD.
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