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HUMAN<EM > POST MORTEM </EM>BRAIN TISSUE – AN INDISPENSABLE SOURCE TO STUDY BRAIN ORGANIZATION
Sarah Wölfleand 2 co-authors
Ulm University
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-387
Presentation
Date TBA
Board: PS06-09PM-387
Event Information
Poster Board
PS06-09PM-387
Poster
View posterAbstract
Despite well-known anatomical and cellular differences, knowledge on human brain architecture and protein distribution is mostly based on rodent studies. This is mostly due to the restricted access to human tissue and/or difficulties in immunohistochemical assays after long-term fixation of archival brains. The aim was to establish high resolution fluorescence microscopy in human post mortem tissue from the gross anatomy course (part of the medical education) to study protein conservation. The included body donors had given their consent to scientific use during their lifetime, an ethics vote is active.
Immunofluorescence staining (IF) was performed on human post mortem brain tissue and antibodies for various synaptic, cellular, and disease-associated proteins were established. For the acquisition of larger 3D volumes, the antigen retrieval capabilities of the tissue clearing technique CLARITY were assessed.
Human post mortem tissue was suitable for a plethora of staining and imaging methods: In total, a list of 30 antibodies was established for human brain studies. In particular, presynaptic VGLUT1 IF intensity allowed the separation of all hippocampal layers in the human hippocampus. CLARITY improved staining quality for some targets. Using the established tools, differences in postsynaptic SHANK2 protein expression were uncovered in comparison to the mouse brain.
Human post mortem brain tissue from the gross anatomy course is a valuable source to study the composition of cells and synaptic proteins in a coherent 3D volume, which is lost with destructive biochemical methods, but indispensable to keep the anatomical context.
Immunofluorescence staining (IF) was performed on human post mortem brain tissue and antibodies for various synaptic, cellular, and disease-associated proteins were established. For the acquisition of larger 3D volumes, the antigen retrieval capabilities of the tissue clearing technique CLARITY were assessed.
Human post mortem tissue was suitable for a plethora of staining and imaging methods: In total, a list of 30 antibodies was established for human brain studies. In particular, presynaptic VGLUT1 IF intensity allowed the separation of all hippocampal layers in the human hippocampus. CLARITY improved staining quality for some targets. Using the established tools, differences in postsynaptic SHANK2 protein expression were uncovered in comparison to the mouse brain.
Human post mortem brain tissue from the gross anatomy course is a valuable source to study the composition of cells and synaptic proteins in a coherent 3D volume, which is lost with destructive biochemical methods, but indispensable to keep the anatomical context.
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