AlphaFold for gene structure annotation

Protein-structure prediction can help distinguish competing gene models. Studies of two fungi and an apicoplast compare original and manually curated annotations, finding that structure-derived scores favour curated models when predictions differ in structured domains. These signals complement sequence-domain analysis but offer little help for uncertainty in potentially disordered regions. The presentation also examines Protenix-mini as a faster alternative retaining discriminatory power, complete-proteome scoring, and a NextFlow pipeline intended to make the workflow usable. It considers applications when transcriptomic evidence is limited and the remaining limitations of structure-based annotation.