c-Fos brain connectome in mouse with chronic corneal pain
Jian Huang, Flora Maguelone, Lisa Amar, Youenn Travert-Jouanneau, Felipe Cybis Perira, Sophie Pezet, Gisella Vetere, Laurence Bourgeais-Rambur, Annabelle Réaux-Le Goazigo · FENS Forum 2024
Dry eye disease (DED) affects 30% of the adult population worldwide and causes corneal pain which remains a therapeutic challenge. Our study aimed to identify the first brain connectome associated with corneal pain by mapping c-Fos positive cells (an indirect marker of neuronal activation) in the whole mouse brain.Adult male C57BL/6 mice received unilateral topical instillation of 0.2% benzalkonium chloride twice/day for 7 days (DED model, n=5) and were compared to naive animals (n=5). Spontaneous ocular pain (eye closure ratio) and corneal mechanical sensitivity (von Frey test) were monitored
ePoster · Neuroscience