Topic: Drp1 phosphorylation

Brain-Derived Neurotrophic Factor (BDNF) is an important regulator of synaptic activity and a mediator of LTP of hippocampal synapses. Furthermore, synaptic activity controls mitochondrial fission and dynamic control of fission regulates plasticity induction in hippocampus. Given the important role of mitochondrial fission in establishing LTP and knowing the key role of BDNF in LTP induction, we hypothesized that the effects of BDNF on synaptic potentiation could be mediated by mitochondrial fission. We observed that local protein synthesis of Drp1, a key mediator of mitochondrial fission, is

The goal of this work is to analyze the mitochondrial changes that Rotenone (Rot) treatment can induce in primary cortical neurons that could contribute to the schizophrenia-like phenotype animal model, previously demonstrated by our research group. For this, we previously determined the Rotenone (Rot) concentration in the cortex (1.325nM) from our animal model with a schizophrenia-like phenotype. Then, we treated the primary cortical neurons with Rot 1.325nM for 24h and investigated: the Rot cellular viability treatment by MTT and CytotoxGlo assay; CI enzymatic activity; oxygen consumption ra