Topic: human brain microvascular endothelial cells

Physiologically relevant human in vitro models of the BBB are essential to improve our understanding of its function in health and disease, identify novel drug targets, and assess potential new therapies. We present a BBB-on-a-chip model comprising human brain microvascular endothelial cells (HBMECs) cultured in a microfluidic platform that allows parallel culture of 40 chips. In each chip, a perfused HBMEC vessel was grown against an extracellular matrix gel in a membrane-free manner. BBBs-on-chips were exposed to varying concentrations of pro-inflammatory cytokines tumor necrosis factor alph

Increased levels of TNF-Related Weak Inducer of Apoptosis (TWEAK) are associated with neuroinflammation, especially during multiple sclerosis (MS), and the cytokine engenders permeabilization of in vitro blood-brain barrier (BBB) models. We sought to determine the effect of TWEAK on human brain microvascular endothelial cells (BMECs), specifically the hCMEC/D3 cell line. We used microarray analysis to investigate the transcriptomic changes of hCMEC/D3 induced by rhTWEAK incubation at three timepoints (3h, 12h, and 24h). We identified a set of differentially expressed genes (DEGs) probing the d