Neuroprotective and antioxidant effects of oxotremorine‑M, a non‑selective muscarinic acetylcholine receptors agonist, in a cellular model of Alzheimer disease
Miriana Scordino, Giulia Urone, Monica Frinchi, Domenico Nuzzo, Costanza Giardina, Marta Di Carlo, Giuseppa Mudò, Valentina Di Liberto · FENS Forum 2024
Oxidative stress and neuroinflammation are crucial phenomena in the pathogenesis of Alzheimer disease (AD). Oxotremorine-M (Oxo), a non-selective muscarinic acetylcholine receptors agonist, exerts neurotrophic functions in primary neurons and modulates oxidative stress and neuroinflammation phenomena in rat brain. Based on this, our study investigates Oxo positive effects on differentiated SH-SY5Y neuroblastoma cells, revealing that Oxo treatment a) enhances cell survival, b) increases neurite length and c) upregulates HSP70 and pHSF1 expression. Moreover, Oxo counteracts tert–Butyl hydroperox