Topic: phospho-proteomics

Neurological diseases are complex network-level processes where many aspects of subcellular function undergo depletion, stress, and sometimes catastrophic decline. Conventional single-molecule studies have had limited success in uncovering these highly interconnected events. Our study utilizes network-level modeling with data from mass spectrometry and targeted data from proximity ligation assay to study processes in various synapses of neurological disorders. We aim to measure synaptic signaling across multiple readouts, stimuli, and genetic/aging contexts and use this to build an atlas and m

Spinocerebellar ataxia 14 (SCA14) is a rare autosomal dominant neurodegenerative disease caused by mutations in protein kinase C gamma gene (PRKCG, PKCγ protein) leading to motor deficits and cognitive decline. PKCγ is a signalling protein strongly expressed in Purkinje cells (PCs) of the cerebellum and is known to be involved in the regulation of synapse formation and PC dendritic development. The effects of PKCγ are mediated by phosphorylation of target proteins which regulate cytoskeleton dynamics playing a critical role in dendritic development. However, the interaction of PKCγ with cytosk