Topic: premyelinating oligodendrocytes

ePoster: 2 ePosters

Dissecting the role of autophagy to elucidate the differential response of oligodendrocytes and astrocytes to hypoxic injury in vitro

Sagar Tyagi, Vadanya Shrivastava, Devanjan Dey, JB Sharma, JK Palanichamy, S Sinha, P Seth, S Sen · FENS Forum 2024

Hypoxic injury in developing glial cells and their differential response can result in neurological deficits in neonates. This study attempts to understand the role of autophagy in developing premyelinating oligodendrocytes (pre-OL), mature-OL and astrocytes exposed to hypoxic injury. Human fetal neural stem cell-derived pre-OL, mature OL and astrocytes were exposed to hypoxia (0.2% oxygen) and normoxia (20% oxygen) for 48 hours followed by RNA-sequencing. Transcriptomic analysis revealed upregulation of autophagy in mature OL and astrocytes but not in pre-OL. MO3.13 (resembling pre-OL) and SV

ePoster · Neuroscience

Evaluating the effect of astrocyte-derived cholesterol on oligodendroglial maturation in an in vitro model of hypoxic brain injury

Vadanya Shrivastava, Devanjan Dey, Sagar Tyagi, Archna Singh, Jai Bhagwan Sharma, Jayanth Kumar Palanichamy, Pankaj Seth, Sudip Sen · FENS Forum 2024

In preterm infants, hypoxic injury to premyelinating oligodendrocytes leads to failure of oligodendroglial maturation causing long-term neuromotor disabilities. As astrocytes influence oligodendrocyte maturation, this study aimed to analyze the transcriptomic changes in astrocytes after hypoxic injury, to identify possible pathways influencing myelination. Human fetal neural stem cell-derived astrocytes, expressing astrocyte-specific glial fibrillary-acidic protein (GFAP) and excitatory amino acid transporters (EAAT1 and EAAT2), were exposed to hypoxia (0.2% oxygen) and normoxia (20% oxygen) f

ePoster · Neuroscience