Topic: RNA interference

Caenorhabditis elegans must distinguish pathogens from nutritious food sources among the many bacteria to which it is exposed in its environment1. Here we show that a single exposure to purified small RNAs isolated from pathogenic Pseudomonas aeruginosa (PA14) is sufficient to induce pathogen avoidance in the treated worms and in four subsequent generations of progeny. The RNA interference (RNAi) and PIWI-interacting RNA (piRNA) pathways, the germline and the ASI neuron are all required for avoidance behaviour induced by bacterial small RNAs, and for the transgenerational inheritance of this b

Dopamine plays a crucial role in the neural mechanisms underlying behavior, yet quantifying its impact through genetic modifications presents significant challenges, particularly due to the high-dimensional nature of the resulting data. In this study, we address these challenges by proposing a novel methodological framework using the Random Forest algorithm, tailored for robust multivariate analysis without the typical assumptions required in conventional statistical testing. Our experimental approach involved detailed video recordings of Drosophila melanogaster larvae, focusing on the effects